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MCherry is a monomeric red fluorescent protein evolved from DsRed. Due to its color, monomeric stability, and low cytotoxicity, mCherry is widely used for molecular labeling and localization of cellular components, with its maximum excitation and emission wavelengths at 587nm and 610nm, respectively.
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After the firefly luciferase mRNA enters the cell, it will express the firefly luciferase protein, which catalyzes the oxidation of D-luciferin in an ATP-dependent manner, producing fluorescence at a wavelength of 560nm. The intensity of the light can reflect the expression level of luciferase.
(Science and Technology Daily) Although researchers have made significant progress in the development of molecular therapies in recent years, the number of newly discovered active substances remains insufficient. Now, the DNA-encoded chemical library (DEL) technology, jointly developed by Harvard University in the US and ETH Zurich in Switzerland, offers a new solution. The technology can automate the synthesis and testing of billions of compounds in a matter of weeks, and can also be used to produce larger drug molecules that will act on targets that are difficult to reach with traditional small molecules.
Key words:numberprotein.
ATP (adenosine triphosphate) is one of the products of biological oxidation and is central to the release, storage and utilization of energy in living organisms.ATP contains two high-energy phosphoric acid bonds and emits at least 30kJ/mol of free energy on hydrolysis.
Key words:protein.cellline
